Coherent Raman Scattering (CRS) microscopy allows noninvasive 3D imaging of live cells based on the vibrational contrast intrinsic to a cell's molecular species. Through improvements in detection sensitivity, theoretical understanding of the contrast mechanism, and development of new laser sources, CRS microscopy, including coherent anti-Stokes Raman scattering (CARS) and Stimulated Raman Scattering (SRS) microscopies have matured as powerful methodologies for bio-imaging. This training course will reflect the state-of-the-art of these rapidly evolving techniques, providing understanding of the underlying principles of nonlinear coherent microscopy, and hands-on experience with CRS in tandem with confocal microscopy. Trainees may bring their own samples to be tested on June 27.

Application

Applicants from academia, government, and industry of all countries are welcome. Slots are available for a maximum of forty trainees. A limited number of fellowships will be granted to graduate students or postdoctoral fellows, courtesy of the sponsors. Application deadline: March 19, 2010.

2010 CRS Workshop Application

Additional CRS 2010 Workshop Information

Lectures

• Professor Sunney Xie, Harvard University: Coherent Raman Imaging: Coming of Age ____________ ________________________________ Stimulated Raman Scattering Microscopy
• Professor Eric Potma, University of California, Irvine: Seeking the Sensitivity Limit
• Dr. Kevin Yiwei Jia, Olympus: Confocal Microscopy
• Mr. Brian Saar, Harvard University: Building CARS a Microscope
• Professor Ji-Xin Cheng, Purdue University: Biological Applications of Coherent Raman Scattering Imaging
• Professor Geoff Young, Harvard Medical School: Intraoperative Coherent Ram Microendoscopy Clinical Applications - 2009
• Dr. Gary Holtom, Harvard University: Ultrafast Lasers for Coherent Raman Microscopy
• Mr. Christian Freudiger, Harvard University: Upgrading Your CARS Microscope to Do SRS
• Dr. Meng Wang, Massachusetts General Hospital: High-throughput Genome-wide Screening for Lipid Regulatory Genes with SRS Microscopy
• Professor Richard Guy, University of Bath: Probing Drug Delivery to the Skin Using CARS
  

Hands-on Training in:

Raman Spectrometry; Laser Scanning Confocal Microscopy; Ultrafast Laser Systems: Mode-locked Lasers, OPO, Autocorrelator, Wavemeter; Details of CRS microscopy: Intensity & Size Dependence of Signal, Epi-detection, Background Rejection, CRS Spectra, Applications of C RS microscopy: Lipids, Live Cells & Tissue Imaging

Additional Information:

Please contact CRS Workshop administrator Teri Howard at howard@chemistry.harvard.edu.

Sponsors:
NSF, APE, Calmar Laser, Chroma, Clark-MXR, Inc., Coherent, High Q Laser, Leica, Olympus, TOPTICA, Zeiss, and the Harvard University Center for Nanoscale Systems.