Coherent Raman Scattering (CRS) microscopy allows noninvasive 3D imaging of live cells based on the vibrational contrast intrinsic to a cell's molecular species. Through improvements in detection sensitivity, theoretical understanding of the contrast mechanism, and development of new laser sources, CRS microscopy, including coherent anti-Stokes Raman scattering (CARS) and Stimulated Raman Scattering (SRS) microscopies have matured as powerful methodologies for bio-imaging. This training course will reflect the state-of-the-art of these rapidly evolving techniques, providing understanding of the underlying principles of nonlinear coherent microscopy, and hands-on experience with CRS in tandem with confocal microscopy. Trainees may bring their own samples to be tested on June 27th (maximum of 8 places available). As of April 15, 2009, Sample Day is full!

Application

Applicants from academia, government, and industry of all countries are welcome. Slots are available for a maximum of forty trainees. A limited number of fellowships will be granted to graduate students or postdoctoral fellows, courtesy of the sponsors. Application deadline: March 20, 2009.

2009 CRS Workshop Application

Additional CRS 2009 Workshop Information

Lectures

• Professor Sunney Xie, Harvard University: Coherent Raman Imaging: Coming of Age ____________ ________________________________ Background-free Imaging with SRS Microscopy
• Professor Eric Potma, University of California, Irvine: CARS Instrumentation: Seeking the Sensitivity Limit
• Dr. Kevin Yiwei Jia, Olympus: Confocal Microscopy
• Mr. Brian Saar, Harvard University: Building CARS a Microscope
• Professor Ji-Xin Cheng, Purdue University: Applications of CRS Microscopy to Neuroscience, Drug Delivery and Obesity Related Disorders
• Professor Geoff Young, Harvard Medical School: Imaging Brain Structures with CRS Microscopy
• Professor Jing Kang, Harvard Medical School: SRS Lipidomics
• Dr. Jason Tsai, Pfizer R&D: Application of 3D, Label-free Optical Imaging in Drug Development - Stimulated Scattering Imaging as a Target Biomarker in Dermatology
• Dr. Gary Holtom, Harvard University: Mode-locked Lasers for CRS Microscopy
• Mr. Christian Freudiger: Upgrading Your CARS Microscope to SRS
  

Hands-on Training in:

Raman Spectrometry; Laser Scanning Confocal Microscopy; Ultrafast Laser Systems: Mode-locked Lasers, OPO, Autocorrelator, Wavemeter; Details of CARS microscopy: Intensity & Size Dependence of Signal, Epi-detection, Background Rejection, CARS Spectra, Applications of CARS microscopy: Lipids, Live Cells & Tissue Imaging

Additional Information:

Please contact CRS Workshop administrator Teri Howard at howard@chemistry.harvard.edu.

Sponsors:
NSF, APE, Calmar Laser, Chroma, Clark-MXR, Inc., Coherent, High Q Laser, Leica, Olympus, TOPTICA, Zeiss, and the Harvard University Center for Nanoscale Systems.